Restriction Enzyme Reference 1.0
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ABOUT Restriction Enzyme Reference
Restriction Enzyme Reference Description: Use this application to search Type IIs restriction enzymes by name, recognition sequence, and overhand. The data is collected from REBase (Nucl.Acids Res. 2007, 35:269-270) and New England BioLabs (NEB, http://www.neb.com), which covers all commercially available Type IIs restriction enzymes with extensive enzyme properties tested by NEB. Features: - Search by enzyme name, recognition sequence, or overhand (sticky end). - Screen through most common Type IIs restriction enzymes with initial index. - Cut site with color-labeled double strain DNA sequences with cut position and two sub-sequences to be separated. Enzyme Properties include: - Enzyme name - Cut site with color-labeled double strain DNA sequences with cut position and two sub-sequences to be separated - Recognition sequence - Overhang - Isoschizomers which share the same recognition sequences - Methylation sensitivity in recognition sequence and their cleavage effects - Buffer and percentage activity of enzymes in different buffers - Diluent buffers - Is time-saver digestion which specify if enzyme is powerful enough to digest in 5 minutes and pure enough for overnight incubation - Incubation temperature for digestion and heat inactivation, and incubation time. - Is recombinant enzyme and original microoganism source - Suppliers - Reference